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Nutritional requirements of Lactobacillus delbrueckii subsp. lactis in a chemically defined medium.

Hébert EM, Raya RR, de Giori GS

Centro de Referencia para Lactobacilos (CERELA)-CONICET, Chacabuco 145, 4000 S. M. de Tucumán, Argentina. ehebert@cerela.org.ar

This study was undertaken to determine the nutritional requirements of Lactobacillus delbrueckii subsp. lactis and to develop a minimal chemically defined medium that supports sustained growth of these microorganisms. The single-omission technique was applied to each component of complete chemically defined medium in order to determine the nutritional requirements. L. delbrueckii subsp. lactis was prototrophic for alanine, glycine, aspartic acid, asparagine, glutamine, threonine, and proline. The lysine requirement was strain-dependent. Magnesium was the only essential oligoelement. These microorganisms also required uracil and guanine and adenine as pyrimidine and purine sources, respectively. In view of the nutritional requirements we designed a new minimal defined medium which supports sustained growth of L. delbrueckii subsp. lactis. This medium is simple and well defined, and should be preferable to complex media for conducting future biochemical, physiological, and genetic studies on L. delbrueckii subsp. lactis.

Published 15 October 2004 in Curr Microbiol, 49(5): 341-5.
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