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Heterogeneity of putative surface layer proteins in Lactobacillus helveticus.

Gatti M, Rossetti L, Fornasari ME, Lazzi C, Giraffa G, Neviani E

Department of Genetic Anthropology Evolution, Parco Area delle Scienze 11A, University of Parma, 43100 Parma, Italy. monica.gatti@unipr.it

The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridization. This study demonstrates S-layer gene variability in the species L. helveticus.

Published 4 November 2005 in Appl Environ Microbiol, 71(11): 7582-8.
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