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Bioactivity quantification of crude bacteriocin solutions.

Delgado A, Brito D, Fevereiro P, Tenreiro R, Peres C

Instituto de Tecnologia Química e Biológica, Department de Microbiology, Av. da República, Quinta do Marquês 2780-505 Oeiras, Portugal. ameliad@itqb.unl.pt

Bacteriocin activity can be quantified by a simple parallel line model based on an agar diffusion assay. The quotient between the inhibition zone area and the sensitivity of the indicator bacterium can be used to compare different bacteriocins. Deferred calculations of relative potencies are possible, once purified compounds are obtained.

Published 12 April 2005 in J Microbiol Methods, 62(1): 121-4.
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