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Demineralization of red crab shell waste by lactic acid fermentation.

Jung WJ, Kuk JH, Kim KY, Park RD

Glucosamine Saccharide Materials-National Research Laboratory (GSM-NRL), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University, Gwangju, 500-757, Korea.

Lactic acid fermentation was applied to demineralize red crab shell waste using Lactobacillus paracasei subsp. tolerans KCTC-3074. Various concentrations (0, 2.5, 5.0, 10.0%) of glucose were supplemented as an initial carbon source and various amounts (2.5, 5.0, 10.0%) of the bacterial culture were inoculated as a starter. Microbial growth was very dependent on glucose concentration but little dependent on inoculum level. The pH decreased rapidly from pH 8 to pH 6 during the first day, at all three inoculum levels. At day 5 of fermentation, the 2.5, 5.0, and 10.0% inoculum levels with 10% glucose supply gave pH 5.5, 5.1, and 4.6, respectively, i.e. a decrease in pH concomitant with an increase in the inoculum level. The total titratable acidities (TTA) at day 5 for 2.5, 5.0, and 10.0% inoculum levels with 10% glucose supplement were 3.1, 4.5, and 8.3%, and the relative residual ash contents were 26.6, 25.9, and 19.0%, respectively. A negative relationship was found between pH and demineralization level (r2 = 0.8571), but there was a positive relationship between TTA and demineralization level (r2 = 0.5532).

Published 21 June 2005 in Appl Microbiol Biotechnol, 67(6): 851-4.
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